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Cells were washed, colleted with 1 mM EDTA in PBS, pelleted (500 g for 5 min) and re-suspended in isolation medium (320 mM sucrose, 10 mM TrisHCl, 1 mM EDTA, pH 7.4)

ROS production during the aging process has been associated with p38 MAPK, JNK, and ERK activities (Zhang et al., 2015)

Aging and Skin Health As we age, our natural glutathione production declines, which can make the skin more prone to damage from UV exposure and environmental pollutants

doi: 10.1007/s00011-007-7056-8

The anti-inflammatory effects of n-3 PUFA are based on their ability to decrease endogenous concentrations of ROS and expression of NF-kB and promote activation of genes involved in antioxidant protection

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