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If IGF-1 remains below the mid-normal range, increase by 0.050.1 mg nightly

For all constructs, filtered supernatants were loaded onto a cleaned and equilibrated 5 mL Roche cOmplete His column (Millipore Sigma Catalog Number: 6781535001) for immobilized metal affinity capture using a benchtop Cole Parmer Masterflex LS peristaltic pump (Catalog Number: EW-07522-20)