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Materials and methods Strains and culture conditions Yeasts were maintained and grown either in YPD broth [1% yeast extract (Oxoid), 2% bacteriological peptone (Oxoid), 2% D-glucose], or in YNB broth [0.69% yeast nitrogen base without amino acids (Formedium), 2% D-glucose] supplemented as required with amino acids, uracil, 5-FOA (Melford), D-histidine (Sigma) or reduced GSH (Sigma)

Gluten- and casein-free dietary intervention for autism spectrum conditions

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Moreover, these foods have a high content of additives (emulsifiers, colorings, sweeteners, preservatives) and other industrial ingredients (such as hydrolyzed proteins, hydrogenated or de-esterified oils), used to make the final product more palatable [185]

82 It has also been discovered that variations in the amino acid sequences, 83 transcription, and efficiency of this gene secretion affect the amounts of vacuolating activity

Gamma-butyrobetaine dioxygenase is promiscuous in substrate selectivity and it processes a number of modified substrates, including the natural catalytic products L-carnitine and D-carnitine, forming 3-dehydrocarnitine and trimethylaminoacetone