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IV glutathione supports the neuroprotective capacity that oral supplementation cannot replicate

Immunostaining Deparaffinized tissues were unmasked using antigen unmasking solution (Vector Laboratories, Burlingame, CA, USA) in a microwave oven

Etienne M.C., et al

Biochem Mol Med

Membranes were then incubated with one of the following primary antibodies overnight at 4 C: AQP3 (1:1000, Santa Cruz Biotechnology, TX, USA), AQP4 (1:1000, Abcam Biochemicals, Grand Island, NY, USA), claudin-1 (1:1000, Invitrogen Technology, Carlsbad, CA, USA), occludin (1:1000, Invitrogen Technology, Carlsbad, CA, USA), Beclin-1 (1:1000, Cell Signaling Technology, Danvers, MA, USA), Atg5 (1:5000, Abcam Biochemicals, Grand Island, NY, USA), iFABP (1:1000, Santa Cruz Biotechnology, TX, USA), HSP70 (1:1000, Enzo Life Sciences, Inc., NY, USA), LC3 (1:1000, Sigma Aldrich) or -actin (1:5000, Sigma Aldrich)

doi:10.1016/j.urology.2004.04.009