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After 3 washes with PBS, the cells were incubated with Alexa Fluor 546-conjugated goat-anti rabbit IgG (Invitrogen, CA, USA) for 1 h at room temperature and then washed again with PBS

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Hypoxia-Inducible Factor-1 Activates the Transforming Growth Factor-/SMAD3 Pathway in Kidney Tubular Epithelial Cells

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