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For quality assurance and quality control, molecular negative template controls (NTC) consisting of PCR-grade water (Invitrogen, Waltham, MA, USA) along with positive controls created by linearized plasmids were run on every qPCR plate

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This is the strict liability principle under the WADA Code

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Let the alcohol dry fully before going in skin that's still damp from the swab will sting more than it needs to