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If exposure of the cells to polysucrose is deemed undesirable (there is evidence that this macromolecule can adsorb to the surface of leukocytes) then centrifuge the blood at 200 gav for 15- 20 min at 18-22C and harvest the buffy coat in the plasma supernatant from the top of the packed erythrocytes and use this in Step 4
Chitosan NPs for infection control Chitosan, composed of -(14) N-acetyl glucosamine and D-glucosamine repeating units, possesses native amine groups that are protonated at physiological pH
The cumulative effect of many missed doses will reduce overall efficacy
This doesnt mean it isnt safe or effective - this simply means there havent been studies (or the roughly $3 million in fees) submitted to the FDA for their review
